home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9409c.zip
/
M9490424.TXT
< prev
next >
Wrap
Text File
|
1994-09-19
|
2KB
|
39 lines
Document 0424
DOCN M9490424
TI Human immunodeficiency virus type 1 Rev is required in vivo for binding
of poly(A)-binding protein to Rev-dependent RNAs.
DT 9411
AU Campbell LH; Borg KT; Haines JK; Moon RT; Schoenberg DR; Arrigo SJ;
Department of Microbiology and Immunology, Medical University of; South
Carolina, Charleston 29425-2230.
SO J Virol. 1994 Sep;68(9):5433-8. Unique Identifier : AIDSLINE
MED/94335055
AB In the absence of Rev or the Rev-responsive element, the Rev-dependent
human immunodeficiency virus type 1 (HIV-1) RNAs do not behave as mRNAs;
rather, they exhibit nuclear defects in splicing and/or nuclear export
and cytoplasmic defects in stability and translation. A translational
initiation factor, eIF-5A, has recently been shown to bind specifically
to the Rev activation domain. As the binding of poly(A)-binding protein
1 (PAB1) to the poly(A) tail of mRNAs is involved in both the stability
and translation of cytoplasmic mRNAs, we investigated whether Rev might
influence the association of PAB1 with cytoplasmic HIV-1 RNAs.
Antibodies were generated against PAB1. We used these antibodies in an
immunoprecipitation assay to detect specific binding of PAB1 to
cytoplasmic mRNAs. We found that in the presence of Rev, PAB1 was
associated with Rev-dependent and Rev-independent RNAs in the cytoplasm
of transfected cells. However, in the absence of functional Rev, we
found little or no PAB1 associated with Rev-dependent RNAs. These RNAs
were capable of binding PAB1 in vitro. These results demonstrate that
HIV-1 RNAs are defective in PAB1 association in the absence of Rev.
DE Amino Acid Sequence Base Sequence Cell Line Cytoplasm/METABOLISM
*Gene Expression Regulation, Viral Gene Products, rev/*METABOLISM
Human HIV-1/*GENETICS In Vitro Molecular Sequence Data
Oligonucleotide Probes/CHEMISTRY Peptides/CHEMISTRY RNA-Binding
Proteins/*METABOLISM RNA, Messenger/METABOLISM RNA, Viral/METABOLISM
Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Translation,
Genetic JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).